Diluent for bca assay

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August undefined, 2024

Web23235 Micro BCA Protein Assay Kit, sufficient reagents for 480 tube assays or 3200 microplate assays . Kit Contents: Micro BCA Reagent A (MA), 240mL . Micro BCA Reagent B (MB), 240mL . ... several clean vials, preferably using a diluent that is similar to the sample buffer. Each 1mL ampule of 2.0mg/mL Albumin WebApr 13, 2024 · Mouse CXCL13/BLC/BCA-1 Catalog Number: DY470 (15 plates) INTENDED USE For the development of sandwich ELISAs to measure natural and recombinant mouse B-Lymphocyte Chemoattractant/B Cell-Attracting Chemokine 1 (BLC/BCA-1). The Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and …

BCA Protein Assay Kit Manual - cosmobiousa.com

WebCayman’s Protein Determination (BCA) Kit is a quantitative assay that can be used with various buffers, water, plasma, serum, urine, and cell lysates. The assay ... with 480 μl of the same diluent as the sample(s) to make a 2 mg/ml BSA Standard Solution. Prepare the BSA Standard dilutions according to Table 1 (see page 7). Webcuvette assay, and a 250 µl microplate assay. The linear range of these assays for BSA is 125–1,000 µg/ml, whereas with gamma-globulin the linear range is 125–1,500 µg/ml. 2. Remove the 1x dye reagent from 4°C storage and let it warm to ambient temperature. Invert the 1x dye reagent a few times before use. 3. javascript programiz online

Protein Assay Data Analysis Thermo Fisher Scientific - US

WebPreparation of BCA working reagent Table 2 below gives guidelines for preparing the BCA working reagent for both the test tube and microtiter assays. Working reagent is prepared by mixing 50 parts BCA solution with 1 part of 4% Cupric Sulfate. Note: The volume of BCA working reagent for the test tube assay is based on 1 ml of reagent per reaction. WebThe BCA assay is based on the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium and the highly sensitive and selective colorimetric detection of the cuprous cation using bicinchoninic acid (BCA).1 Disulfide reducing agents, ... Diluent . µ. L) BSA Source and Concentration ... WebThe Micro BCA Assay uses concentrated solutions and extended incubation tim es for the detection of dilute protein samples. The amount of protein present in a solution can be quantified by ... Diluent (ml) Final Concentration (µg/ml) A 300µl from Stock 5.7 100 B 3ml from Tube A 3 50 C 3ml from Tube B 3 25 D 3ml from Tube C 3 12.5 ... javascript print image from url

Smallest volume for BCA Assay : r/labrats - Reddit

BCA diluent and standard curve - Protein and Proteomics

WebThe bicinchoninic acid (BCA) assay is one of many ways to measure the total protein concentration of an unknown sample. In this assay, copper (II) [Cu2+] is reduced to copper (I) [Cu1+] in the presence of peptides in alkaline solutions. BCA can chelate (bind to) Cu1+, creating a purple color with a maximum absorbance at 562 nm. As the protein ... WebApr 13, 2024 · Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and plasma samples. The Reagent Diluent selected for use can alter the performance of an immunoassay. Reagent Diluent optimization for samples with complex matrices such as serum and plasma, may improve their performance in this assay. javascript print image base64Web23225 BCA Protein Assay Reagent Kit, sufficient reagents for 500 test tube or 5,000 microplate assays ... preferably using the same diluent as your sample. Each 1 ml ampule of 2.0 mg/ml Albumin Standard is sufficient to prepare a set of diluted standards for either working range suggested in Table 1. There will be sufficient volume javascript praca junior

"WebIs this dilution valid or do you ignore the 50 ul of assay diluent since each sample is treated that way? 2. As stated above, this ELISA assay begins with 50 ul of assay diluent in each well, to ... " - Diluent for bca assay

Diluent for bca assay

BCA Protein Assay Kit (Test Tube) - Reducing Agent …

WebApr 11, 2024 · This assay recognizes natural and recombinant human BLC/BCA-1. The factors listed below were prepared at 50 ng/mL in calibrator diluent and assayed for cross-reactivity. Preparations of the following factors at 50 ng/mL in a mid-range recombinant human BLC/BCA-1 control were assayed for interference. No significant cross-reactivity or WebModule and the BCA Protein Assay is 5 µg/mL (Figure 1) and the maximum is 2 mg/mL (Figure 2). The Enhanced Protocol requires a 60°C incubation for 30 minutes. The Standard Protocol permits either a 30-minute incubation at 37°C or a 2 hour-incubation at room temperature. Figure 1. BCA Enhanced Protocol assay was performed on the Modulus …

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WebThe Thermo Scientific ™ Pierce BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric ... (BSA) ampule into …

WebDec 7, 2024 · The provided Antigen/Antibody Diluent Buffer contains 0.01% Thimerosal. Additional protease inhibitors can be added if required. This kit is sold based on number of tests. A ‘test’ simply refers to a single assay well. The number of wells that contain sample, control or standard will vary by product. Review the protocol completely to WebBioVision’s BCA Assay Kit II (cat. # K813) and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation ... Prepare BSA Standards as suggested in the table below by diluting BSA Standard using de-ionized water or same diluent (with or without the reducing agent)* as that of protein samples. Other similar dilutions can ...

WebFeb 1, 2024 · Abcam’s BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in … WebSample Diluent Concentrate 2 (2X) Catalog #: DYC002. 4 Citations 2 Reviews. Submit a Review. Datasheet / COA / SDS. Catalog #. Availability. Size / Price. Qty.

WebBCA Assay Introduction This protocol is adapted from ThermoFisher Scientific Pierce BCA Protein Assay Kit. Here the Microplate reader method (small volume 10-25 µL) is used to quantify the total protein ... Vial Volume of Diluent [µL] Volume and Source of BSA [µL] Final BSA concentration [µg/mL] A 0 300 of Stock 2000 B 125 375 of Stock 1500

Web23225 BCA Protein Assay Reagent Kit, sufficient reagents for 500 test tube or 5,000 microplate assays ... preferably using the same diluent as your sample. Each 1 ml … javascript pptx to htmlWebI want to load 50 ug/20 ul/well of SDS-PAGE for Western blot and I have protein concentrations 6.18, 4.9, 5.76, 6.53, 4.43, 5.83, 5.11& 7.46 ug/ul. Suppose I calculate … javascript progress bar animationhttp://wolfson.huji.ac.il/purification/PDF/Protein_Quantification/PIERCE_BCA_KIT.pdf javascript programs in javatpointWeb1. The Pierce BCA assay reagents can also be purchased as a kit, catalog # 23225. 2. BSA standards should be made using 2 mg/ml BSA stock solution(vials provided in the BCA … javascript programsWebAssay Protocol BQC BCA Protein Quantification Kit Microplate 1. Prepare the calibrate in 1.5 mL tubes following the Table 3. For the diluent use the same buffer as in the samples. 2. Pipette 10 μL of each standard and unknown sample replicate into a microplate well. 3. Add 200 μL of freshly prepared BCA Working Solution javascript print object as jsonWebFeb 1, 2024 · Abcam’s BCA protein assay kit reducing agent compatible (microplate) (ab207003) measures total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents. ... water or an appropriate diluent to a concentration within the assay range (25-2000 μg/mL). For unknown samples, it is recommended to use three ... javascript projects for portfolio redditWebApr 12, 2024 · Tubes were pierced with a hot needle and centrifuged at 14,000xg for 10 min at 4°C to collect the lysate. Protein concentration in the lysate was measured by BCA Assay (Thermo Fisher Scientific, #22662). Proteins were reduced with 10 mM DTT (Sigma, 1h, 56°C) and subsequently with 55 mM iodoacetamide (Sigma, 1h, 25°C, dark). javascript powerpoint